Single plattform Heidelberg
In the province of Quebec (QC) and according to the Laboratoire de santé publique du Québec (LSPQ) surveillance program, . Between 20, 70% of QC isolates exhibited pulsotype 2 (SHXAI.0001/SHBNI.0001).Approximately 23% of the isolates were isolated from blood, supporting the increased invasive rate associated with this serovar, compared to 7% for serovar Typhimurium.
This is not an indication of a security issue such as a virus or attack.
Thirteen background clinical isolates were added to the study, 11 of which had the same PFGE pattern (SHEXAI.0001/SHEBNI.0001) and PTs 18, 19, 26, and 29; 1 isolate with a closely related PFGE pattern (SHXAI.0009/SHBNI.0025); and 1 isolate with a highly variant PFGE pattern (SHXAI.0197/SHBNI.0077).
The subtyping and metadata for the isolates used in this study are summarized in Table 1. Heidelberg isolates was performed at the PHAC-NML Core Genomics facility.
In this study, we assessed the usefulness of a high-quality core genome single-nucleotide variant (hq SNV) analysis to distinguish between three epidemiologically defined outbreaks typed by PFGE and sharing the most commonly occurring PFGE pattern (SHEXAI.0001/SHEBNI.0001) of The isolates included in this study were collected through the provincial surveillance program implemented in 2003 to monitor incidences of human salmonellosis, thus enabling detection of rapid clustering and/or outbreaks.
Food isolates were received from the Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec (MAPAQ) Laboratoire d'expertises et d'analyses alimentaires following food-poisoning investigations.
Phylogenetic analysis based on hq SNVs extracted from WGS separated the outbreak isolates into three distinct groups, 100% concordant with the epidemiological data.